天美传媒

Research Article

Absorption Spectral Analysis of Zn2+ or Cu2+ Coordination with Human Serum Albumin using Zincon

Research Core for Interdisciplinary Sciences, Okayama University, Okayama, Japan

*Corresponding Author:

Koji Matsuura
Cardiovascular Physiology
Graduate School of Medicine
Dentistry and Pharmaceutical Sciences
Okayama University, 3-1-1 Tsushima-naka
Kita, Okayama 700-8530, Japan
Tel: +81-86-251-8610
Fax: +81-86-251-8610
E-mail: kojimatu@md.okayama-u.ac.jp

Received date: August 29, 2014; Accepted date: September 25, 2014; Published date: September 30, 2014

Citation: Matsuura K and Sugimoto I (2014) Absorption Spectral Analysis of Zn²⁺ or Cu²⁺ Coordination with Human Serum Albumin using Zincon. J Anal Bioanal Tech 5:209 doi: 10.4172/2155-9872.1000209

Copyright: © 2014 Matsuura K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Human serum albumin (HSA) is used for mammalian embryo culture in assisted reproductive technology (ART) to adjust osmotic pressure and growth factor concentration in culture media. In human serum, transition metal ions, particularly Zn²⁺, bind to the coordination sites of HSA; thus analyzing the binding of these metal ions may help predict the possible harmful effects of these metals on embryo culture. In this study, we found that metal ions and zincon bound to HSA, and that a complex was formed with the HSA molecule. Using this method, the metals that bind to the multi-binding site of HSA, particularly Zn²⁺ can be evaluated by colorimetry in an ART laboratory. This analytical method is potentially applicable for metal detection in HSA in ART clinics and also for quick analysis of the coordination of ⁶⁴Cu²⁺ with HSA as a probe chemical in positron emission tomography.

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