天美传媒

Abstract

Biomolecular chirality plays a critical role in the structure and function of biological molecules, influencing processes from enzyme activity to drug interactions. Circular Dichroism (CD) spectroscopy has emerged as a pivotal analytical technique for probing chiral properties of biomolecules. Recent advancements in CD spectroscopy have significantly enhanced its sensitivity, resolution, and applicability. Innovations such as synchrotron radiation CD (SRCD), laserbased CD, and computational methods for data analysis have expanded the capabilities of this technique. These developments allow for more detailed and accurate assessments of secondary and tertiary structures of proteins, nucleic acids, and other chiral biomolecules. Additionally, the integration of CD spectroscopy with complementary techniques like X-ray crystallography and NMR spectroscopy provides a more comprehensive understanding of biomolecular dynamics and interactions. This review highlights the latest technological advancements in CD spectroscopy, explores their applications in structural biology and drug discovery, and discusses future directions for this evolving field. By shedding light on the intricate world of biomolecular chirality, these advancements hold promise for novel insights and innovations in biomedical research.