Research Article
Altered Monoamine Metabolism in High Fat Diet Induced Neuropsychiatric Changes in Rats
| Dhirendra Pratap Singh1,2, Kanthi Kiran Kondepudi1, Mahendra Bishnoi1* and Kanwaljit Chopra2 | |
| 1National Agri-food Biotechnology Institute (NABI), S.A.S. Nagar, Punjab, India, 160071 | |
| 2University Institute of Pharmaceutical Sciences (UIPS), Punjab University, Chandigarh, India, 160014 | |
| Corresponding Author : | Dr. Mahendra Bishnoi, Ph.D. Scientist-C, Department of Nutritional Science & Technology National Agri-Food Biotechnology Institute (NABI) (Department of Biotechnology, Government of India) C-127, Industrial Area, Phase VIII, S.A.S. Nagar (Mohali), Punjab, India-160071 Tel: +91-172-2290128 Fax: +91-172-4604888 E-mail: mbishnoi@gmail.com |
| Received September 08, 2014; Accepted October 20, 2014; Published October 25, 2014 | |
| Citation: Singh DP, Kondepudi KK, Bishnoi M, Chopra K (2014) Altered Monoamine Metabolism in High Fat Diet Induced Neuropsychiatric Changes in Rats. J Obes Weight Loss Ther 4:234. doi:10.4172/2165-7904.1000234 | |
| Copyright: © 2014 Singh DP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
Abstract
Objective: To investigate the role of central and peripheral monoamine metabolism on neuropsychological status in high fat diet induced obese rats. Method: Animals were fed on high fat diet for 12 weeks. Periodically blood was collected to estimate serum Metanephrine (MN) levels. After 11 weeks, behavioural paradigms of depression, locomotor activity and cognition were performed. Central (brain) and peripheral i.e. Visceral White Adipose Tissue (vWAT) and Brown Adipose Tissue (BAT) MN levels, Monoamine Oxidase A and B (MAO-A, MAO-B) enzyme activity and Acetylcholinesterase (AChE) activity was estimated. Results: High fat diet significantly increased the body weight which was negatively correlated with the serum metanephrine concentration. However, metanephrine concentration in brain was significantly decreased whereas it significantly increased in vWAT with no change in BAT. In obese animals, immobility time in forced swim test and transfer latency in elevated plus maze was significantly increased while locomotor activity was significantly decreased. Central MAO-A and MAO-B activities were increased while enzyme activities showed a significant reduction in vWAT with no change in BAT. Brain AChE activity was also increased significantly in obese rats. Conclusion: Metabolism of biogenic monoamines plays a critical role in altered neuropsychiatric behaviours associated with diet induced obesity.
