Research Article
Development and Validation of Analytical Method by RP-HPLC for Quantification of Alpha-Mangostin Encapsulated in PLGA Microspheres
Aimen Abdo Elsaid Ali, Muhammad Taher, Helaluddin ABM and Farahidah Mohamed*
Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Malaysia
- *Corresponding Author:
- Farahidah Mohamed
Department of Pharmaceutical Technology
Kulliyyah of Pharmacy
International Islamic University Malaysia
Jalan Istana, Bandar Indera Mahkota
25200 Kuantan, Pahang, Malaysia
E-mail: farahidah@iium.edu.my
Received date: September 21, 2012; Accepted date: November 26, 2012; Published date: December 03, 2012
Citation: Ali AAE, Taher M, Helaluddin ABM, Mohamed F (2012) Development and Validation of Analytical Method by RP-HPLC for Quantification of Alpha- Mangostin Encapsulated in PLGA Microspheres. J Anal Bioanal Tech 3:155. doi: 10.4172/2155-9872.1000155
Copyright: © 2012 Ali AAE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited..
Abstract
A simple, rapid, precise and highly accurate RP-HPLC method was developed and validated for determination of alpha-mangostin content extracted from PLGA-microspheres. Method was developed using a silica-based deactivated C-18 column (4.6×100 mm, 3 μm) with a mobile phase of 70-80 % v/v acetonitrile (A) and 0.1% v/v orthophosphoric acid (B), with the following pre-determined timed-gradient program: 70% (A) isocratic for 6 min, 70-75% (A) in 1.2 min, 75-80% (A) in 0.4 min, 80% (A) isocratic for 2.4 min, 80-70% (A) in 0.4 min, finally 70% (A) isocratic for 5 min, with a flow rate of 1 mL/min, detected at 320 nm by a UV detector. Linearity was obtained over the range of 1-200 μg/mL with r2=0.9995. The precision was achieved based on repeatability and intermediate with RSD of 0.13-0.6% and 0.57-1.2%, respectively. Percent recovery of 100.55% to 103.82% with RSD 0.086 - 0.15 implied high accuracy of the method. Limit of detection and limit of quantitation were 0.038 and 0.121 μg/ml, respectively suggesting good sensitivity of the method. The method is envisaged to be effectively used for routine assay for encapsulated alpha-mangostin in PLGA microspheres.
