Research Article
Fabrication of a Dual-Enzyme Sensing System Based on Surface Plasmon Resonance Imager (SPRi) for Simultaneous Determination of Bio-Markers
Yuhei Hida1 and Hiroaki Shinohara1,2*
1Graduate School of Innovative Life Science for Education, University of Toyama, 3190 Gofuku, Toyama, Japan
2Graduate School of Science and Engineering for Research, University of Toyama, 3190 Gofuku, Toyama, Japan
- *Corresponding Author:
- Hiroaki Shinohara
Graduate School of Innovative Life Science for Education
University of Toyama, 3190 Gofuku
Toyama 930-8555, Japan
Tel: +81 076 445 6832
Fax: +81 076 445 6832
E-mail: hshinoha@eng.u-toyama.ac.jp
Received date: August 31, 2015; Accepted date: September 21, 2015; Published date: September 28, 2015
Citation: Hida Y, Shinohara H (2015) Fabrication of a Dual-Enzyme Sensing System Based on Surface Plasmon Resonance Imager (SPRi) for Simultaneous Determination of Bio-Markers. J Anal Bioanal Tech 6:279. doi:10.4172/2155-9872.1000279
Copyright: © 2015 Hida Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Saccharides such as glucose, galactose and amino acids like lysine, phenylalanine are known as bio-markers of metabolic disorders, and several analytical methods have been developed to determine these bio-markers in blood and urine by enzymatic method, HPLC analysis and so on. Multi-biosensing systems for simultaneous determination of these bio-markers are presently expected to be necessary for troublesome diagnosis. We have previously proposed enzyme-sensors based on a two-dimensional surface plasmon resonance imager (2D-SPRi), which can sensitively monitor refractive index change of local region in the evanescent field on an Au chip. L-lysine sensor, L-glutamate sensor and galactose sensor has been developed each by combining oxidase reactions with 2D-SPR imaging of Os polymer-redox state. In this study, we aimed to develop a dual-enzyme sensor capable of the simultaneous detection of lysine and galactose by means of co-immobilizing LysOx/Os-HRP polymer spot and GalOx/Os-HRP polymer spot adjacently on the Au chip, which is divided into two sections.
