Research Article
Speciation of Intracellular Zn, Fe and Cu within both iPS Cells and Differentiated Cells Using HPLC Coupled to ICP-MS
Akihiro Arakawa1, Nobuaki Shiraki2, Tomonori Tsuyama3, Shoen Kume2, Daigo Iwahata1* and Naoyuki Yamada1
1Institute for Innovation, Ajinomoto Co. Inc., Kanagawa, Japan
2Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa, Japan
3Divisions of Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan
- *Corresponding Author:
- Daigo Iwahata
Institute for Innovation
Ajinomoto Co. Inc., Kanagawa, Japan
Tel: +81442444428
Fax: +81442117609
E-mail: daigo_iwahata@ajinomoto.com
Received Date: December 17, 2015; Accepted Date: December 28, 2015; Published Date: January 04, 2016
Citation: Arakawa A, Shiraki N, Tsuyama T, Kume S, Iwahata D, et al. (2016) Speciation of Intracellular Zn, Fe and Cu within both iPS Cells and Differentiated Cells Using HPLC Coupled to ICP-MS. J Anal Bioanal Tech 7:295. doi:10.4172/2155-9872.1000295
Copyright: © 2016 Arakawa A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Zinc, iron and copper are essential mineral nutrients for the human body to function. It is also known that there are biological processes which relate to metals in the body including cells. Therefore, understanding about intracellular metals is important for the regeneration medicine. Intracellular metals were thought to be able to be divided into two forms by function: i) protein binding metals and ii) labile metals. However, it is difficult to quantify the concentration in both intracellular metal forms. In this study, the analytical method including the pre-treatment and HPLC coupled to ICP-MS (HPLC-ICP-MS) techniques was developed. Our method is able to fractionate two forms of intracellular metals and quantify each concentration, simultaneously. Finally, the presented method was applied to analysis of several cell states. As a result, dynamic changes of intracellular metal concentration were demonstrated during cell differentiation from iPS cells to pancreatic β cells.
