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In oil reservoir, hydrogen sulfide is produced by sulfate reducing bacteria which has nasty odour. The production of hydrogen
sulfide is called as reservoir souring. It is a common problem during secondary oil recovery when water is injected to
produce the remaining oil from the oil reservoir. In the presence of electron donor in injection water, sulfate reducing bacteria
(SRB) can reduce sulfate into sulfide. For the control of sulfide production, broad spectrum biocides can be used which control
the microbial process. Use of nitrate as a biocide, directly affect the SRB population growth by numerous competitive and
inhibitory mechanisms. The mechanism of souring control by nitate and biocide have been evaluated from oil field simulating
sand packed columns. In our study, to simulate the oil reservoir environment, four up-flow sand packed-bed columns was
used. To simulate waterflooding, synthetic modified Colville Synthetic Brine (CSB) media were pumped in the column and
columns was inoculated with produced water sample of oil field. When nitrate was added in the CSB media, souring control
was reported at injection port but not at outlet port due to total consumption of nitrate at the inlet port, so that inclusion of
nitrate inhibits the SRB activity in inlet zone. In our study, Benzalkonium Chloride (BAC) is also used as biocide in reservoirsimulating
columns in exclusively and combination way. BAC is involved in a gradual decrease in sulfide concentration.
Continuous low nitrate and BAC concentration resultant was complete inhibition of SRB activity. Thus it was appeared that
nitrate in combination with biocide is synergistic effect.
Biography
Ganshyam Prajapat has completed his Master’s degree in Microbiology from Central University of Rajasthan. He is currently pursuing his PhD from Central University of Rajasthan. His area of interest is in microbial souring, corrosion, control of biosouring, new biocide treatment and cyanobacterial UV-protecting molecules.