Activation of NADPH Oxidase Complex by ISA Virus Nucleoprotein Overexpression: Alteration of SUMO-1 Protein Levels a Consequence of Cellular Oxidative Stress

Fernanda Fredericksen; Melina Villalba; Valentina Arriagada; Francisco Torres; Nicolas Maldonado; Juan K Romero; V铆ctor H Olavarr铆a

Activation of NADPH Oxidase Complex by ISA Virus Nucleoprotein Overexpression: Alteration of SUMO-1 Protein Levels a Consequence of Cellular Oxidative Stress

Fernanda Fredericksen, Melina Villalba, Valentina Arriagada, Francisco Torres, Nicolas Maldonado, Juan K Romero and V脙颅ctor H Olavarr脙颅a^*: Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Campus Isla Teja S/N, Valdivia, Chile

^*Corresponding Author : V脙颅ctor H Olavarr脙颅a, Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Campus Isla Teja S/N, Valdivia, Chile, Email: volavarria@uach.cl

Received Date: Dec 18, 2017 / Accepted Date: Dec 20, 2018 / Published Date: Dec 27, 2017

Abstract

The pathogenic mechanism of the infectious salmon virus (ISAv) remains unknown. One methodological approach for solving this unknown is to understand the roles of each viral component separately. Therefore, the present study evaluated the viral nucleoprotein (NP) of ISAv to establish effects to reactive oxygen species (ROS) production and SUMOylation profile balance. Salmon head kidney-1 cells transfected with NP evidenced a strong respiratory burst activation and the genic induction of p47phox, SOD, GLURED, and Bad. Additionally, NPtransfected VERO cells showed alteration in the profile of SUMOylated proteins. Notably, pharmacological inhibition of the NADPH oxidase complex through apocynin blocked ROS production and high NP-mediated cellular ROS levels. These results suggest that the ISAv NP alone can trigger, in transfected cells, a strong production of ROS (able to activate pro-apoptotic marker expressions) and modifications to the post-translational profile mediated by SUMO-1.

Keywords: Reactive oxygen species; SUMOylation; VERO cells; Apocynin

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Citation: Fredericksen F, Villalba M, Arriagada V, Torres F, Maldonado N, et al. (2017) Activation of NADPH Oxidase Complex by ISA Virus Nucleoprotein Overexpression: Alteration of SUMO-1 Protein Levels a Consequence of Cellular Oxidative Stress. J Mol Immunol 3: 116.

Copyright: © 2017 Fredericksen F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Journal of Molecular Immunology
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