Immune Gene Expression during Bovine Herpesvirus-1 Infection of MDBK Cells: Molecular Characterization of Interferon-Stimulated Gene 15

Cristopher Stepke; Fernanda Fredericksen; Valentina Arriagada; Jorge Oltra; Cristian Toledo; Melina Villalba; V铆ctor H Olavarr铆a

Immune Gene Expression during Bovine Herpesvirus-1 Infection of MDBK Cells: Molecular Characterization of Interferon-Stimulated Gene 15

Cristopher Stepke¹, Fernanda Fredericksen¹, Valentina Arriagada¹, Jorge Oltra², Cristian Toledo², Melina Villalba¹ and V脙颅ctor H Olavarr脙颅a¹^*: ¹Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Campus Isla Teja S/N, Valdivia, Chile; ²Servicio Agrícola y Ganadero Los Ríos, , Ejército Libertador 1271, Río Bueno, Chile

^*Corresponding Author : V脙颅ctor H Olavarr脙颅a, Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Campus Isla Teja S/N, Valdivia, Chile, Email: volavarria@uach.cl

Received Date: Dec 18, 2017 / Accepted Date: Jan 01, 2018 / Published Date: Jan 08, 2018

Abstract

Bovine herpesvirus 1 (BoHV-1) infection extensively modulates host-cell gene expressions. To further understandings of BoHV-1 pathogenesis, Madin-Darby bovine kidney cells were infected, and RT-qPCR expression analyses were performed. Apart from classical immune genes (e.g. PKR, Mx1, IRF7, CCR4, TXNIP), EGR3, IFITM3, and ISG15 presented notably high transcripts. To gain further insight, the bovine ISG15 gene was characterized and compared against human, mouse, dog, fugu, salmon, and zebrafish homologues. In silico analysis revealed several conserved regions of interest, including a set of potential transcriptional motifs in the proximal 5’-untranslated region for all analyzed species. A protein-deduced bovine ISG15 sequence also evidenced many conserved domains in humans. Finally, in vitro infection assays with bovine viral virus-1 or BoHV-1 both resulted in elevated blood ISG15 levels. Collectively, the obtained data indicate that ISG15 could potentially serve as an early immune marker during BoHV-1 and bovine viral diarrhea virus-1 infection.

Keywords: BoHV-1; BVDV-1; ISG15; Immune marker

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Citation: Stepke C, Fredericksen F, Arriagada V, Oltra J, Toledo C, et al. (2018) Immune Gene Expression during Bovine Herpesvirus-1 Infection of MDBK Cells: Molecular Characterization of Interferon-Stimulated Gene 15. J Mol Immunol 3: 117.

Copyright: © 2017 Stepke C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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Journal of Molecular Immunology
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