Case Report
A Strategy for GC/MS Quantification of Polar Compounds via their Silylated Surrogates: Silylation and Quantification of Biological Amino Acids
Marco Guida1, Maria Michela Salvatore1 and Francesco Salvatore2*
1Dipartimento di Biologia, Università degli Studi di Napoli “Federico II”, via Cintia, 21, Napoli, Italy
2Dipartimento di Scienze Chimiche, Università degli Studi di Napoli “Federico II”, via Cintia, 21, Napoli, Italy
- *Corresponding Author:
- Francesco Salvatore
Dipartimento di Scienze Chimiche
University of Naples “Federico II”
Via Cintia, 21-80126 Napoli, Italy
Tel: 39 081 674389
E-mail: frsalvat@unina.it
Received date: July 19, 2015; Accepted date: July 28, 2015; Published date: August 04, 2015
Citation: Guida M, Salvatore MM, Salvatore F (2015) A Strategy for GC/MS Quantification of Polar Compounds via their Silylated Surrogates: Silylation and Quantification of Biological Amino Acids. J Anal Bioanal Tech 6:263 doi:10.4172/2155-9872.1000263
Copyright: © 2015 Salvatore F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Substitution of polar functionalized compounds with silylated (e.g., trimethylsilylated) surrogates prior to GC/ MS analysis is a widely used analytical strategy. Calibration is a most demanding step of this strategy. In fact, a calibration function is usually acquired by converting known amounts of the pure analyte to its silylated surrogate using the same conditions employed for processing unknown samples. The cumbersome need of acquiring a new calibration function prevents, to a large extend, the possibility of modifying silylation and instrumental settings on a sample by sample basis as would be appropriate in a number of cases. The modified standard additions calibration method, suggested in this paper, overcomes this difficulty by integrating in a single analytical procedure calibration and sample analysis. Furthermore, the suggested procedure compensates for matrix effects which may be a serious source of inaccuracy and is a tool that can be used during method development in order to find the most suitable silylation conditions for a given analyte. The implementation and benefits of the modified standard additions calibration method are explored in this paper on the basis of a symbolic but enlightening experiment dealing with the very representative GC/MS quantification of biological amino acids via their trimethylsilylated derivatives.
