Research Article
Biodegradation and Decolorization of Acid Red by Acinetobacter radioresistens
| Mohandass Ramya *, Iyappan S, Manju A and Jiffe John S | |
| Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur 603203, India | |
| Corresponding Author : | Ramya Mohandass Department of Genetic engineering School of Bioengineering SRM University Kattankulathur 603203, India E-mail: ramya.mohandass@gmail.com |
| Received August 12, 2010; Accepted September 28, 2010; Published October 02, 2010 | |
| Citation: Ramya M, Iyappan S,ManjuA, Jiffe JS (2010) Biodegradation and Decolorization of Acid Red by Acinetobacter radioresistens. J Bioremed Biodegrad 1:105. doi: 10.4172/2155-6199.1000105 | |
| Copyright: © 2010 Ramya M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Acid red decolorizing bacteria was isolated and identified as Acinetobacter radioresistens.The effect of operation parameters such as medium composition, pH, temperature, dye concentration on the decolorization of acid red was studied and the products of degradation were analyzed and confirmed using LC–MS analysis. The reductive cleavage of azo bond was catalyzed by azoreductase, the key enzyme for the azo dye degradation. The catalytic reduction of acid red 37 by purified azoreductase in the presence of NADH as electron donor was studied and the products of degradation were determined as 1-{3-amino-5-[(aminoxy)sulfonyl]phenyl}ethanol and 7,8-diamino-3[(aminoxy)sulfonyl]naphthalene-1-ol.
