Research Article
Determination by UPLC/MS-MS of Coenzyme Q10 (CoQ10) in Plasma of Healthy Volunteers before and after Oral Intake of Food Supplements Containing CoQ10
Giacomo Luca Visconti1, Lara Mazzoleni1, Chiara Rusconi2, Vittorio Grazioli2, Gabriella Roda1*, Giorgio Manini3 and Veniero Gambaro11Dipartimento di Scienze Farmaceutiche, Sezione di Chimica Farmaceutica "Pietro Pratesi", Università degli Studi di Milano, Via L. Mangiagalli 25, 20133, Milano, Italy
2Centro Diagnostico Italiano, Via Saint Bon, 20, 20147 Milano, Italy
3Scharper S.p.A., Viale Ortles, 12, 20139 - Milano, Italy
- *Corresponding Author:
- Gabriella Roda
Dipartimento di Scienze Farmaceutiche
Sezione di Chimica Farmaceutica "Pietro Pratesi"
Università degli Studi di Milano, Via L. Mangiagalli 25
20133, Milano, Italy,
Tel: 390250319065;
E-mail: gabriella.roda@unimi.it
Received date: November 24, 2015 Accepted date: December 09, 2015 Published date: December 14, 2015
Citation: Visconti GL, Mazzoleni L, Rusconi C, Grazioli V, Roda G, et al. (2015) Determination by UPLC/MS-MS of Coenzyme Q10 (CoQ10) in Plasma of Healthy Volunteers before and after Oral Intake of Food Supplements Containing CoQ10. J Anal Bioanal Tech S13:011. doi:10.4172/2155-9872.S13-011
Copyright: © 2015 Visconti GL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
To evaluate CoQ10 concentration a UPLC/MS-MS method for its analysis in plasma samples was developed. Before validation, the choice of the anticoagulant, of the Internal Standard (CoQ9; IS) and the stability of CoQ10 in plasma were evaluated. These determinations were necessary to assess the effect of the oral intake of CoQ10 in nine healthy volunteers after 10 days of treatment with a food supplement containing it. The basal levels of CoQ10 were determined and the analyses were repeated after four days to take into account the variability of endogenous substances. After oral intake, the increase in plasma concentration of CoQ10 resulted statistically significant (P<0.05), indicating that the assumption of food supplements containing this ubiquinone could be of help to increase its concentration, thus exploiting the its beneficial properties.
