Research Article
Development and Validation of a Simple Method for the Detection of Fascaplysin in Plasma
Kenneth H Swartz1, Frederick A Valeriote1, Joseph Media1, Tyler A Johnson2, Karen Tenney2, Phillip Crews2 and Jiajiu Shaw3*
1Department of Internal Medicine, Henry Ford Health System, Detroit, Michigan, USA
2Department of Chemistry and Biochemistry and Institute for Marine Sciences, University of California, Santa Cruz, California, USA
321st Century Therapeutics Inc., Ferndale, Michigan, USA
- *Corresponding Author:
- Jiajiu Shaw
21st Century Therapeutics Inc.
Ferndale, Michigan 48220, USA
Tel: 1-248-545-0595
Fax: 1-248-545-0595
E-mail: jiajiushaw@gmail.com
Received date: September 27, 2012; Accepted date: October 23, 2012; Published date: October 29, 2012
Citation: Swartz KH, Valeriote FA, Media J, Johnson TA, Tenney K, et al. (2012) Development and Validation of a Simple Method for the Detection of Fascaplysin in Plasma. J Anal Bioanal Tech 3:150. doi: 10.4172/2155-9872.1000150
Copyright: © 2012 Swartz KH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Fascaplysin is a cytotoxic natural product isolated from a variety of Indo-Pacific marine organisms, primarily Fascaplysinopsis sponges and Didemnum tunicates. Positive xenograft studies involving this alkaloid structural class have indicated that fascaplysin may serve as an important lead compound for preclinical development. This study was undertaken as a prelude to a full pharmacokinetics and therapeutic assessment of fascaplysin. We describe here a simple plasma preparation and a rapid HPLC method for the detection of fascaplysin in mice. The method was validated by parameters including good linear correlation, a limit of quantification of 107.1 μg/mL, and a good precision with a coefficient of variation of 0.92% for 10 μg/mL. This method provides excellent sensitivity and visualization of fascaplysin as a single peak allowing for rapid analysis of plasma samples involving absorption, distribution, and studies. A preliminary pharmacokinetics study in C57Bl/6 mice using 20.6 mg/kg fascaplysin yielded a biphasic curve with T½α=16.7 min, T½β=11.7 h, and C0 of 17.1 μg/mL.
