Research Article
Genotyping of Bacillus anthracis Strains Circulating in Albania
Ardi Peculi1, Emanuele Campese2, Luigina Serrecchia2, Leonardo Marino2, JonidaBoci1, Bizena Bijo1, Alessia Affuso2, Valentina Mercurio2, Luigi Giangrossi2 and Antonio Fasanella2*
1Instituti I SigurisëUshqimoredhe Veterinarisë, Rruga Aleksandër Moisiu 82, Tiranë, Albania, Italy
2Istituto Zoo profilattico Sperimentale of Puglia and Basilicata, Anthrax Reference Centre of Italy, via Manfredonia 20, Foggia, Italy
- *Corresponding Author:
- Dr. Antonio Fasanella
Istituto Zoo profilattico Sperimentale of Puglia and Basilicata
Anthrax Reference Centre of Italy
via Manfredonia 20, Foggia, Italy
Tel: +39 080 405 78
E-mail: a.fasanella@izsfg.it
Received Date: November 26, 2015; Accepted Date: January 31, 2015; Published Date: February 08, 2015
Citation: Peculi A, Campese E, Serrecchia L, Marino L, Boci J, et al. (2015) Genotyping of Bacillus anthracis Strains Circulating in Albania. J Bioterror Biodef 7:131. doi: 10.4172/2157-2526.1000131
Copyright: © 2015 Peculi A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Abstract
Anthrax in Albania is an endemic disease characterized by few outbreaks involving a very low number of animals. Nineteen samples of soil coming from burial sites and 11 strains of Bacillus spp isolated from died animals from different districts of Albania were examined. The analysis of soil samples revealed that 11 of them were contaminated with anthrax spores, while only 8 strains were confirmed as Bacillus anthracis. The analysis of CanSNPs showed that all isolates belong to lineage A major subgroup A.Br.008/009 (Trans-Eurasian or TEA strains). The MLVA test at 15 loci showed three different genotypes: Albania GT/1, Albania GT/2 and Albania GT/3. However all the genotypes are genetically very similar to each other which confirm the hypothesis that all of them are the result of the evolution of a local common ancestral strain. Two distinct genotypes (Albania GT/2 and Albania GT/3) were found in the same burial site in the district of Kukës. Although in the scientific literature there have been cases in which two different genotypes were found in the same carcass, the authors believe that in this case the isolation of two different genotypes could be justified by the presence in the same burial sites of two or more animals that died in different times and as a consequence of different outbreaks.
