天美传媒

Abstract

Objective: Evidence suggests that low serum levels of vitamin D may increase the severity of Osteoarthritis (OA). VDR gene polymorphism is known for its association with osteoporosis. The inverse relationship between osteoporosis and OA suggests that VDR gene polymorphism is a candidate gene to be associated with OA. This study was done to analyze the association of vitamin D receptor gene polymorphism (Taq1 and Apa I) and serum vitamin D levels in knee osteoarthritis Methods: This case control study consisted of 180 Osteoarthritis Knee patients and 150 controls. Cases were clinically diagnosed according to ACR criteria. Gradation of the disease was done by using KL grading system on the basis of radiological findings. The serum levels of vitamin D were assessed by using kit of Enzyme Linked Immunosorbent Assay. Detection of VDR gene polymorphisms (Taq1 and Apa I) were done by PCR-RFLP technique Results: We observed an insignificant association for genotypes of TaqI(p=0.086) and ApaI(p=0.60) polymorphism between cases and controls.However, for TaqI marginal significant association (p=0.053, OR 1.4, 95% CI 1.008-1.945) was observed between wild type(T) allele and mutant type(t) allele, but for Apa I, there was no significant difference (p=0.334, OR 1.17, CI 1.367-1.867) between wild type(A) allele and mutant type(a) allele.We observed a significant association of low level of serum vitamin D levels in homozygous mutant(tt) genotypes as compared to heterozygous(Tt) and wild type(TT) genotypes. But In case of ApaI, we found higher serum vitamin D levels in homozygous mutant(aa) genotype in comparison to heterozygous(Aa) and wild type(AA) genotype but this difference was not statistically significant Conclusions: Though insignificant association was found between osteoarthritis and the genotypes of TaqI and ApaI polymorphism, a significant association with mutant allele of TaqI(t) was observed. Additionally, the association of mutant allele with reduced level of vitamin D was noted.

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