Journal of Analytical & Bioanalytical Techniques
天美传媒 Access
Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 天美传媒 Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
天美传媒 Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Citations : 6413
Indexed In
- CAS Source Index (CASSI)
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Academic Journals Database
- 天美传媒 J Gate
- Genamics JournalSeek
- JournalTOCs
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Ulrich's Periodicals Directory
- Electronic Journals Library
- RefSeek
- Directory of Research Journal Indexing (DRJI)
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- Scholarsteer
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Euro Pub
- ICMJE
Useful Links
Related Subjects
Share This Page
Determination of amlodipine in human plasma by liquid chromatography芒�?�?tandem mass spectrometry and its application to pharmacokinetic & bioequivalence studies
6th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Syed N Alvi, Rajaa F Hussein, Reem Al Swayeh, Saleh Al Dgither and Muhammad M Hammami
King Faisal Specialist Hospital & Research Center, Saudi Arabia
Posters-Accepted Abstracts: J Anal Bioanal Tech
DOI:
Abstract
A rapid Liquid Chromatographic-Tandem Mass Spectrometric (LC-MS/MS) assay for the measurement of amlodipine level in human plasma was developed and validated. Amlodipine and tizanidine (IS) were extracted from plasma using mixture of dichloromethane and tertiary butyl methyl ether (1:3, v:v) and reconstituted with 100 �?录l mixture of methanol and water (1:1, v:v). The mobile phase consisted of acetonitrile and 0.1% formic acid (80:20, v:v). Analysis was performed at room temperature using a reversed phase Atlantis dC18 (2.1x100 mm, 3 �?录m) column. The components of interest were detected in the positive ion mode of electrospray ionization using transition 409.8 芒�?�? 238.4 and 254.3 芒�?�? 43.9 for amlodipine and the IS, respectively. The relationship between amlodipine concentration in plasma and peak height ratio of amlodipine to IS was linear 芒�?楼0.9868 in the range of 0.2芒�?�?20 ng/ ml; intra and inter-day accuracy between 101-114%, and coefficient of variations were 芒�?陇14.4%. The quantification limit of amlodipine in 0.5 ml plasma was 0.2 ng/ml and the detection limit was 0.1 ng/ml. The method was successfully validated and applied in a bioequivalence study of four tablet formulations of amlodipine.Biography
Syed N Alvi obtained his PhD in Chemistry from Osmania University, Hyderabad, India in 2001. He is currently Scientist at King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia. He his research interest includes method development and validation and application for pharmacokinetic and bioequivalence studies.
Email: salvi@kfshrc.edu.sa
