天美传媒

Abstract

Garlic is one of the famous herbal plants which have showed beneficial properties on atherosclerosis risk factors. Some components of garlic suppress cholesterol and triglyceride biosynthesis and its absorptin, resulting in lowering of serum cholesterol and triglycerides and increase in HDL level. However, the mechanism of these specific properties is not fully understood. In the small intestine, ATP-binding cassette transporters G5, G8 and A1 (ABCG5, ABCG8 and ABCA1), as well as Niemann-Pick C1 like 1 (NPC1L1) protein has important roles in cholesterol metabolism. In this study, we evaluated the beneficial effect of aqueous extract of garlic on lipid profile and also expression of npc1l1, abca1, abcg5 and abcg8 genes in the intestine of N-Marry mice fed a high cholesterol diet as a possible mechanism of garlic effect. Mice were randomly divided into three groups (n=8): Group 1: high cholesterol diet (HCD, or obsese) (received chow + 2% cholesterol + 0.5% cholic acid); Group 2: garlic (received chow + 4% (w/w) garlic extract + 2% cholesterol + 0.5% cholic acid); and Group 3: received chow only. After 30 days, mice were anesthetized and blood was collected. The small intestine of mouse was removed, washed and entrocytes were scraped and used for the experiments. Blood factors were measured enzymatically and expression of mRNA levels for the above-mentioned proteins was determined by RT-PCR. Water extract of garlic markedly declined blood lipids (p<0.05), compared with the obsese group. Expression of the intestinal npc1l1 was significantly decreased (p<0.01) in the garlic group, compared with the chow group, while abcg5 (p<0.01), abcg8 (p<0.01) and abca1 (p<0.05) expressions were significantly increased. In conclusion, this experiment shwed a possible mechanism for the beneficial effects of the garlic in declining blood lipids by decreasing the intestinal lipid absorption and increasing excretion of cholesterol back into the intestinal lumen.

Biography

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