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Lawsonia inermis Linn. is an important medicinal
plant, which grows up to 4 to 5 feet high in hot and
dry climates and widely cultivated as a hedge plant. It is
a unique source of various pharmacologically important
compounds such as naphthoquinone (lawsone)
derivatives, aliphatic components, triterpenes, sterols,
phenolic derivatives, coumarins, xanthones, and
flavonoids. As the global scenario is now changing
towards the use of safer non-toxic plant products
having traditional medicinal applications, development
of modern drugs from henna should be emphasized
for the control of various diseases. Lawsonia inermis
is known to have immunomodulatory, antiviral,
antibacterial, antifungal, antifertility, hepatoprotective,
antimitotic, analgesic and anti-inflammatory properties.
Conventionally it is propagated through stem cuttings
or by seeds, which cannot meet out demand of quality
planting material. Therefore, there is a need to develop an
efficient micropropagation protocol for the production
of quality planting material of Lawsonia inermis. In the
present study, effect of cytokinins and polyamines on
multiplication of Lawsonia inermis have been reported.
Multiple shoots of L. inermis were induced using nodal
segment explant on MS medium supplemented with
0.20 mg-1 6-benzylaminopurine (BAP) + 0.2 mgl-1
Kinetin (Kn), 0.5 mg-1. Rate of multiplication was higher
in the medium supplemented with two cytokinins as
compared to the polyamines. There was no significant
difference in the length of shoots in all the combination
used. Rooting was readily achieved upon transferring
the shoots onto MS basal medium supplemented with
2.0 mg-1 indole-3-butyric acid (IBA) after ten days of
culture. Micropropagated plants were acclimatized and
successfully grown in soil and it is expected that this
protocol will be very useful for large-scale production of
Lawsonia inermis in future.
Biography
Prof A Chaudhury, Chairman, Department of Bio & Nano Technology, Guru Jambheshwar
University of Science & Technology, Hisar has teaching and research experience of more
than twenty years in the area of Molecular Biology and Plant Biotechnology. He has published
more than 65 research papers in National and International journals and participated in 25
National and International conferences. Eight students have been awarded PhD degrees
under his Supervision and eight more are working under his Supervision in different areas
of Molecular Biology & Biotechnology. Additionally, he has completed three Research &
Development projects from various funding agencies and PI and Coordinator of DBT
sponsored HRD Program, FIST, BIF, DST sponsored M. Tech. Program and UGC SAP DRS
phase I program. He is a recipient of DBT Overseas Associateship and Commonwealth
Visiting Fellowship, UK and awarded one European Patent and one Indian Patent in Plant
Biotechnology.
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