天美传媒

Abstract

Saliva contains cells and compounds, of local and non-local oral origin, namely inorganic, organic non-protein, protein/ polypeptide,
and lipid molecules. This body fluid offers a non-invasive and stress-free alternative to serum, which can reflect real-time levels of
biomarkers. In the last few years, steroids and non-steroid hormones have been investigated in saliva, providing particularly useful
biomarkers for physical exercise behavior or psychosocial stress evaluation (e.g. cortisol, testosterone, leptin). In recent years, the
attention turned also to the non-hormonal protein components of saliva. Salivary α-amylase is one of the major protein components
of saliva and its main function is the enzymatic digestion of carbohydrates, but it is also important for mucosal immunity in the oral
cavity, as it inhibits the adherence and growth of bacteria. This enzyme is considered a marker for SNS activity and for metabolic
energy balance, sensitive to physical exercise and stress. In this context, its activity/expression in overweight and obesity is of interest.
Saliva proteome is being analyzed by 2DE in several physiological/pathologic situations in order to identify potential biomarkers.
Additionally, saliva can also be useful to follow lipidemic profiles, since a correlation between levels of total cholesterol and HDL has
been demonstrated. Despite the convenient assessment, some characteristics of salivary components also entail some pitfalls that
might complicate study design and interpretation of results. To overcome some of these problems, the establishment of standard
protocols for collection, analysis and expression of results is a priority.

Biography

Ana Rodrigues Costa has completed her PhD in Biochemistry from Évora University in 2012, studying the contribution of Na, K-ATPase to pancreatic B-cell dysfunction in
DT2. She is currently Assistant Professor of Biochemistry in the Department of Chemistry at Evora University, Portugal. She developed investigation in ICT research center,
as Senior Investigator of the Functional Biochemistry group. Her main scientific interests include: i) Physiopathology of DT2, ii) Signal transduction studies in cellular models
of disease; iii) The Identification of salivary biomarkers in pathology.