Journal of Obesity & Weight Loss Therapy
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Influences of Palmitoleic Acid In metabolism and Gene expression of white Adipose tissues cells
17th International Conference on Obesity, Diet and Nutrition
Maysa Mariana Cruz, Farias TM, de Sa RDC, Batini F, Barbosa N and Alonso-Vale MIC
Federal University of Sao Paulo, Brazil
Posters & Accepted Abstracts: J Obes Weight Loss Ther
DOI:
Abstract
We have recently demonstrated that palmitoleic acid (16:1n7) increases lipolysis, glucose uptake and glucose utilization for energy production in white adipose cells. In the present study, we tested the hypothesis that palmitoleic acid modulates bioenergetic activity in white adipocytes. For this, 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes in the presence (or absence) of palmitic (16:0) or palmitoleic (16:1n7) acid at 100 or 200 渭M. The following parameters were evaluated: lipolysis, lipogenesis, fatty acid (FA) oxidation, ATP content, oxygen consumption, mitochondrial mass, citrate synthase activity and protein content of mitochondrial oxidative phosphorylation (OXPHOS) complexes. Treatment with 16:1n7 during 9 days raised basal and isoproterenol-stimulated lipolysis, FA incorporation into triacylglycerol (TAG), FA oxidation, oxygen consumption, protein expression of subunits representing OXPHOS complex II, III, and V and intracellular ATP content. These effects were not observed in adipocytes treated with 16:0. Palmitoleic acid, by concerted action on lipolysis, FA esterification, mitochondrial FA oxidation, oxygen consumption and ATP content, does enhance white adipocyte energy expenditure and may act as local hormone.Biography
Maysa Mariana Cruz will completed her PhD at the age of 28 years from Federal University of São Paulo. She is a pharmacist, Master of science and has published 1 paper as first author and 7 papers as coauthor.
E-mail: maysamariana@gmail.com
