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Abstract

An ethnobotanical survey of 40 individuals in the Wilaya of Mascara identified 40 medicinal plants used for the treatment of diabetes mellitus. Among these plants, Malva sylvestris, olea europaea sylvestris, Citrus aurantium have been selected. The objective of our work is the phytochemical study and the antioxidant activity of the three plants selected. The raw methanolic extracts of Malva sylvestris and their stems, olea europaea sylvestris and Citrus aurantium were obtained by the extraction method: maceration. The quantitative determination of total polyphenols by the Folinâ��Ciocalteu method and the flavonoids in the presence of AlCl3, the total polyphenol content in Malva sylvestris and their stems, olea europaea sylvestris and Citrus aurantium represents 556.33 �¼g EAG/g MS and 443.33 ug EAG/G Ms; 557.66 gEAG/g Ms 447.77 �¼gEAG/gMS. The content of flavonoids is 1032.33 �¼g EQer/g Ms 898.66 ug EQer/g Ms 894.33, �¼g EQer/g Ms 1428.33 �¼gEQer/g MS. Antioxidant activity of the extracts prepared was measured by two methods. The first is the use of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the second is reducing power of FRAP iron. These methods show that these plants have good antioxidant activity. mhanine11@yahoo.fr

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