Journal of Analytical & Bioanalytical Techniques
天美传媒 Access
Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 天美传媒 Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
天美传媒 Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Citations : 6413
Indexed In
- CAS Source Index (CASSI)
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Academic Journals Database
- 天美传媒 J Gate
- Genamics JournalSeek
- JournalTOCs
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Ulrich's Periodicals Directory
- Electronic Journals Library
- RefSeek
- Directory of Research Journal Indexing (DRJI)
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- Scholarsteer
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Euro Pub
- ICMJE
Useful Links
Related Subjects
Share This Page
RP-HPLC method for simultaneous determination of retinol, �?卤-tocopherol, �?鲁-tocopherol, retinolpalmitate and �?虏-carotene in human plasma
6th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Milkica Crevar Saka�?聧, Zorica Vuji�?�?, Bojana Vidovi�?�? and Branka Ivkovi�?�?
University of Belgrade, Serbia
Posters-Accepted Abstracts: J Anal Bioanal Tech
DOI:
Abstract
Simple, isocratic RP-HPLC method with UV detection for measurement of liposoluble vitamins: retinol, �?卤-tocopherol, �?鲁-tocopherol, retinol-palmitate and �?虏-carotene in human plasma was developed. Plasma samples were prepared for analysis by liquid-liquid extraction. Ethanol was used for precipitation of proteins. Further extraction of analytes was performed with n-hexane. Extracts were evaporated to dryness under stream of nitrogen and further reconstituted in ethanol before HPLC analysis. Separation of analytes was performed on Zorbax Eclipse XDB-C18 Rapid Resolution, 4.6x100 mm, 3.5 �?录m chromatographic column with mobile phase consisting of methanol and ethanol (75:25, v/v). Mobile phase flow rate was 1 mL/min. Column temperature was set on 40�?掳C and autosampler temperature on 10�?掳C. Total run time was 16 minutes. UV detection of retinol and retinol-palmitate was performed on 325 nm, �?卤-tocopherol and �?鲁-tocopherol at 292 nm and �?虏 carotene on 450 nm. Tocopherol-acetate was used as internal standard. Calibration curves for all analytes were constructed in following ranges: retinol, �?卤-tocopherol and �?鲁-tocopherol (5芒�?�?35 �?录g/mL), �?虏-carotene and retinol-palmitate (10芒�?�?35 �?录g/mL). Developed method was successfully used for routine determination of liposoluble vitamins in plasma samples of healthy, middle-aged volunteers.Biography
Milkica Crevar Saka�?聧 has completed her PhD at the Department of Medicinal Chemistry, Faculty of Pharmacy, University of Belgrade. She works as Teaching Assistant and Researcher at Department of Medicinal Chemistry.
Email: frodo@pharmacy.bg.ac.rs
