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In Association with
SNP markers to study functional polymorphism in fatty acid desaturases of Indian sesame germplasm
6th World Congress on Biotechnology
Nupur Mondal and K V Bhat
National Bureau of Plant Genetic Resources, India
Posters-Accepted Abstracts: J Biotechnol Biomater
DOI:
Abstract
Sesame (Sesamum indicum L) is one of the oldest and most nutritional oilseed crops whose fatty acid desaturase genes have been poorly understood. A germplasm collection of ninety nine entries comprising sesame (Sesamum indicum L) cultivars, landraces, introgressed lines and wild species were analyzed for the fatty acid compositions of their seed oil. The entries varied widely in their fatty acid compositions. Linoleic acid along with oleic acid comprised a major portion (86%) of total fatty acids. Moreover, we targeted 4.4 Mbp of the genomic DNA of sesame which comprises of stearoyl acyl carrier protein desaturase (sad), fatty acid desaturase 2 (fad2) and omega 3 fatty acid desaturase (o3fad) genes in ninety nine accessions. SNPs and INDELS were found after aligning the sequences. These SNPs were associated to the fatty acid content using TASSEL. 245 associations were identified by GLM when including population structure in the analysis and controlling for multiple testing. Results of this study show that out of 245 only 30 SNPs (Single nucleotide polymorphism) were found to be associated to oleic, linoleic and linolenic acid content. Molecular breeders targeting for increase of particular fatty acid can use our data to genotype the population followed by marker assisted selection and genetic transformation. Genetic transformation of sesame with certain genes involved in fatty acid synthesis will provide a means to effect changes in oil composition.Biography
Nupur Mondal has completed her MSc from University of Delhi and PhD from NBPGR, New Delhi. During her PhD, she has worked on molecular markers in oilseeds. She has worked in NRC Plant Biotechnology, a premier organization where she has worked in rice functional genomics.
Email: nupur.mondal84@gmail.com
