Journal of Analytical & Bioanalytical Techniques
天美传媒 Access
Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 天美传媒 Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
天美传媒 Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Citations : 6413
Indexed In
- CAS Source Index (CASSI)
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Academic Journals Database
- 天美传媒 J Gate
- Genamics JournalSeek
- JournalTOCs
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Ulrich's Periodicals Directory
- Electronic Journals Library
- RefSeek
- Directory of Research Journal Indexing (DRJI)
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- Scholarsteer
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Euro Pub
- ICMJE
Useful Links
Related Subjects
Share This Page
Validation of RP HPLC method for determination of ibandronate sodium in pharmaceutical dosage form
6th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Ivkovi�?�? Branka, �?�?olakovi�?�? Milo�?隆, Crevar-Saka�?聧 Milkica and Vuji�?�? Zorica
University of Belgrade, Serbia
Posters-Accepted Abstracts: J Anal Bioanal Tech
DOI:
Abstract
Ibandronate sodium [(1-hydroxy-3-(methyl pentyl amino) propylidene bisphosphonic acid monosodium monohydrate)] is the sodium salt of ibandronic acid, a synthetic nitrogen-containing bisphosphonate drug. This new, third generation bisphosphonate is used in treatment of bone diseases like Paget芒�?�?s disease, malignant hypercalcemia and postmenopausal osteoporosis. A simple, accurate and sensitive liquid chromatographic method has been developed for the assay of ibandronate sodium in tablet dosage form. The separation was achieved on C18 (250x4.6 mm), 5 �?录m chromatographic column. Mobile phase was mixture of 1 mL ortho-phosphoric acid, 50 mL CH3CN, 0.96 g Na pentansulfonate and 0.1 g Na EDTA in 1000 mL H2O. Column temperature was 40 �?掳C, mobile phase flow rate 1.0 mL/min and detection wavelength 195 nm. The method was validated according to ICH Q2 (R1) requirements. It was proved that method is specific for determination of ibandronate sodium. Linearity was confirmed with calculated r value (r=0.9996). Accuracy was tested at three concentrations levels (80%, 100% and 120%) and confirmed by obtained Recovery values (98.40芒�?�? 99.89%). Precision was tested at two levels: intra-assay precision and intermediate precision. Calculated relative standard deviations were 0.29% and 0.83%, respectively. Small variations of mobile phase composition (proportion of organic solvent, concentration of ion pair reagent) and column temperature did not affect qualitative and quantitative system responses significantly, which proved method芒�?�?s robustness. Applicability of the method was confirmed by analysis of commercially available tablets.Biography
Ivkovi�?�? Branka has completed PhD from University of Belgrade, Faculty of Pharmacy, Department of Pharmaceutical Chemistry.
Email: blucic@pharmacy.bg.ac.rs
