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ISSN: 2153-0777

Journal of Bioengineering and Bioelectronics
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Label-Free Biosensor Cell Assays

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Label-Free Biosensor Cell Assays

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Label-Free Biosensor Cell Assays

Label-free biosensor cell assay converts a cellular response into a signal by using transducer. Depending on the nature of transducers; label-free biosensors used for whole cell sensing are mostly divided into optical- and electric-based.

Label-Free Biosensor Cell Assays related journals
Journal of Biochips and TissueChips, Journal of Molecular Pharmaceutics & Organic Process Research, Journal of Biosensors & Bioelectronics,  Biosensors Journal, Journal of Molecular Biomarkers & Diagnosis, Journal of Biological Engineering, Journal of Micro-Bio Robotics, Journal of Bio- and Tribo-Corrosion, Journal of Industrial Microbiology & Biotechnology, Cellular and Molecular Bioengineering.

Label-Free Biosensor Cell Assays

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    The inflammasome of activated human B-cells regulates IL-1β and IgM: A crosstalk between the innate and the adaptive immune response
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    Modulating the Antioxidant Defences to Potentiate Oncolytic Virotherapy in Refractory Cancer Cells
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    Melatonin sensitizes human breast cancer cells to ionizing radiation
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    Classically, the 3’untranslated region (3’UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3’UTR alone, without all other elements in mRNA such as 5’UTR and coding region. The importance of independent 3’UTR RNA (referred as I3’UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3’UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3’UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3’UTR were important for its tumor suppression activity. Then, the C/EBP 3’UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3’UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3’UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990’s to 2000’s, world scientists found several 3’UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3’UTR regions, although the existence of their transcribed products as independent 3’UTR RNAs is still to be confirmed. Our studies indicate that the independent 3’UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
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  • Fan-Gang Tseng
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    Molecular Profiling of Cancer Cells
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