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Potential role of N-benzylcinnamide in inducing neuronal differentiation from human amniotic fluid mesenchymal stem cells
7th Annual Global Pharma Summit
Wipawan Thangnipon, Nicha Puangmalai, Nirut Suwanna, Rungtip Soi-ampornkul, Ruchee Phonchai, Naiphinich Kotchabhakdi, Sujira Mukda, Tatsanee Phermthai, Suphakde Julavijitphong, Patoomratana Tuchinda and Saksit Nobsathian
Mahidol University, Thailand Kasetsart University, Thailand
Posters & Accepted Abstracts: Clin Pharmacol Biopharm
DOI:
Abstract
Neurodegenerative disorders are characterized by chronic and progressive loss of neurons in structure and function related to aging such as Alzheimer芒�?�?s disease, the latter characterized by the degeneration of cholinergic neurons in basal forebrain connected to the cerebral cortex and hippocampus. Amniotic fluid mesenchymal stem cells (AF-MSCs) have been proposed as one of the candidates for stem cell therapy of nervous system disorders. This study demonstrate that incubation of AF-MSCs, obtained from 16-20 week pregnant women with 10 ng/ml bone morphogenetic protein (BMP)-9 for 48 hours in conditioned medium resulted in trans-differentiation to cholinergic neuronal-like cells. This phenomenon could also be obtained with N-benzylcinnamide (PT-3). Pre-treatment for 1 hour with 10 nM PT-3 augmented BMP-9 trans-differentiation effect, elevated �?虏III-tubulin cell numbers and fluorescence intensity of immunoreactive ChAT, ameliorated BMP-9-related production of reactive oxygen species and enhanced anti-apoptosis status of the neuronal-like cells. The trans-differentiation process was accompanied by increased p53 but decreased Notch1 and SIRT1 (p53 deacetylase) levels and activation of p38, ERK1/2 MAPK and PI3K/Akt pathways, in concert with inactivation of JNK, all of which were accentuated by PT-3 pre-treatment. These findings suggest that N-benzylcinnamide may not provide a useful adjuvant in BMP-9-induced trans-differentiation of AFMSCs into ultimately cholinergic neurons.Biography
Email: wipawan.tha@mahidol.ac.th
