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Clinical Pharmacology & Biopharmaceutics
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Methods in Clinical Pharmacology

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Methods in Clinical Pharmacology

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Methods in Clinical Pharmacology

clinical pharmacology methods are often used that have been developed by other disciplines, such as analytical chemistry ,biochemistry/molecular biology, physiology, psychology.  and Evaluation has become a more and more difficult, expensive and time-consuming process. The effect of a new compound has to be detected by in vitro and in vivo methods of pharmacology.

Journals related to methods in clinical pharmacology
Clinical Pharmacology & Biopharmaceutics, Clinical & Experimental Pharmacology, , Biochemistry & Pharmacology: 天美传媒 Access, The Journal of Clinical Pharmacology, International Journal of Basic & Clinical Pharmacology, Journal of Anaesthesiology Clinical Pharmacology, Clinical Pharmacology: Advances and Applications, Pharmacology and Toxicology, International Journal of Basic & Clinical Pharmacology.

Methods in Clinical Pharmacology

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    Classically, the 3脙垄芒聜卢芒聞垄untranslated region (3脙垄芒聜卢芒聞垄UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3脙垄芒聜卢芒聞垄UTR alone, without all other elements in mRNA such as 5脙垄芒聜卢芒聞垄UTR and coding region. The importance of independent 3脙垄芒聜卢芒聞垄UTR RNA (referred as I3脙垄芒聜卢芒聞垄UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3脙垄芒聜卢芒聞垄UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3脙垄芒聜卢芒聞垄UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3脙垄芒聜卢芒聞垄UTR were important for its tumor suppression activity. Then, the C/EBP 3脙垄芒聜卢芒聞垄UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3脙垄芒聜卢芒聞垄UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3脙垄芒聜卢芒聞垄UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990脙垄芒聜卢芒聞垄s to 2000脙垄芒聜卢芒聞垄s, world scientists found several 3脙垄芒聜卢芒聞垄UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3脙垄芒聜卢芒聞垄UTR regions, although the existence of their transcribed products as independent 3脙垄芒聜卢芒聞垄UTR RNAs is still to be confirmed. Our studies indicate that the independent 3脙垄芒聜卢芒聞垄UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
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